Covalent Binding of Reduced Metabolites of [15N3]TNT to Soil Organic Matter during a Bioremediation Process Analyzed by 15N NMR Spectroscopy

Autor:	Christof Achtnich, Hans-Joachim Knackmuss, Errol Fernandes, Jean-Marc Bollag, Hiltrud Lenke
Rok vydání:	1999
Předmět:	Azoxy chemistry.chemical_classification Chemistry Soil organic matter Nitro compound Soil chemistry General Chemistry Nuclear magnetic resonance spectroscopy complex mixtures chemistry.chemical_compound Humin Environmental Chemistry Humic acid Organic chemistry Organic matter Nuclear chemistry
Zdroj:	Environmental Science & Technology. 33:4448-4456
ISSN:	1520-5851 0013-936X
DOI:	10.1021/es990427+
Popis:	Evidence is presented for the covalent binding of biologically reduced metabolites of 2,4,6-15N3-trinitrotoluene (TNT) to different soil fractions (humic acids, fulvic acids, and humin) using liquid 15N NMR spectroscopy. A silylation procedure was used to release soil organic matter from humin and whole soil for spectroscopic measurements. TNT-contaminated soil was spiked with 2,4,6-15N3-trinitrotoluene and 14C-ring labeled TNT, before treatment in a soil slurry reactor. During the anaerobic/aerobic incubation the amount of radioactivity detected in the fulvic and humic acid fractions did not change significantly (11−16%), whereas the radioactivity bound to humin increased to 71%. The 15N NMR spectra of the fulvic acid samples were dominated by a large peak that corresponded to aliphatic amines or ammonia. In the early stages of incubation, 15N NMR analysis of the humic acids indicated bound azoxy compounds. The signals arising from nitro and azoxy groups disappeared with further anaerobic treatment. At t...
Databáze:	OpenAIRE
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