High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

Autor:	J. Eibl, N Barrett, P. L. Turecek, A Mitterer, F G Falkner, Kistner O, W Bodemer, F Dorner, S Kandels, F Scheiflinger, R T A MacGillivray
Rok vydání:	1992
Předmět:	viruses Chinese hamster ovary cell Hematology Biology biology.organism_classification Virology Molecular biology Virus HeLa chemistry.chemical_compound Thrombin chemistry medicine Vero cell Poxviridae Orthopoxvirus Vaccinia medicine.drug
Zdroj:	Thrombosis and Haemostasis. 68:119-124
ISSN:	2567-689X 0340-6245
Popis:	SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1e85170108f773f064e26b789c1208c8 https://doi.org/10.1055/s-0038-1656335 Zobrazit plný text záznamu