| Autor: |
A M Galan, M. R. Hernández, J. Aznar-Salatti, Juan Carlos Reverter, Gines Escolar, J. Bozzo, M. Pino, Antonio Ordinas, M Vinas |
| Rok vydání: |
2002 |
| Předmět: |
|
| Zdroj: |
Vox Sanguinis. 82:113-118 |
| ISSN: |
0042-9007 |
| DOI: |
10.1046/j.1423-0410.2002.00157.x |
| Popis: |
Background and Objectives We explored the effect on haemostasis of different factor IX (FIX) concentrates under thrombocytopenic conditions using an in vitro perfusion technique. Materials and Methods A moderate experimental thrombocytopenia (25 000–30 000 platelets/µl) was induced by means of a filtration procedure in blood anticoagulated with low-molecular-weight heparin. The effects of three different FIX concentrates – a prothrombin complex concentrate (PCC), an intermediate-purity concentrate (FIX/X), and a high-purity concentrate (HPFIX) – on platelet deposition and fibrin formation on subendothelium were assessed at two different shear rates (600/second and 1200/second). Activation of the coagulation system was monitored through assessment of prothrombin activation fragment 1 + 2 (F1 + 2). Results Fibrin deposition increased after addition of FIX concentrates, but only showed a significant increase in experiments performed after incubation of PCC at the lower shear rate (600/second) (64·25 ± 9·61% vs. control 31·22 ± 8·02%; P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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