Marine invertebrates of the Sea of Okhotsk as a new source of lypopolysaccharide-binding proteins

Autor: M. P. Isaeva, Tamara F. Solov'eva, V. I. Gorbach, G. A. Naberezhnykh, S. I. Bakholdina
Rok vydání: 2014
Předmět:
Zdroj: Russian Journal of Marine Biology. 40:59-65
ISSN: 1608-3377
1063-0740
DOI: 10.1134/s1063074014010027
Popis: Invertebrates from the Sea of Okhotsk were studied as a source of proteins that are capable of binding to lipopolysaccharides (LPSs) of gram-negative bacteria. A DOT analysis using dansyl-labeled LPS that we developed revealed LPS-binding proteins in blood-cell lysates of 21 out of 33 investigated species of invertebrates. Most of the investigated species with positive LPS-binding activity were decapod crustaceans (class Malacostraca, phylum Arthropoda). Hemocyte lysates from the red king crab Paralithodes camtschaticus and the sculptured shrimp Sclerocrangon boreas contained several LPS-binding proteins with different molecular weights. LPS-binding proteins were found for the first time in echinoderms (classes Holothuroidea, Asteroidea, and Echinoidea), sipunculans, and brachiopods.
Databáze: OpenAIRE