Adaptation of aerobic methylobacteria to dichloromethane degradation
| Autor: | Torgonskaia Ml, Nina V. Doronina, Firsova IuE, Trotsenko IuA |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Chromatography
biology Sodium Potassium cyanide chemistry.chemical_element musculoskeletal system biology.organism_classification complex mixtures Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound chemistry cardiovascular system Methylobacterium Sodium azide Methylopila helvetica cardiovascular diseases Methylobacterium extorquens Dehalogenase Dichloromethane |
| Zdroj: | Applied Biochemistry and Microbiology. 43:47-51 |
| ISSN: | 1608-3024 0003-6838 |
| DOI: | 10.1134/s0003683807010085 |
| Popis: | A shortening of the lag phase in dichloromethane (DCM) consumption was observed in the methylobacteria Methylopila helvetica DM6 and Albibacter methylovorans DM10 after prior growth on methanol with the presence of 1.5% NaCI. Neither heat nor acid stress accelerated methylobacterium adaptation to DCM consumption. Sodium azide (1 mM) and potassium cyanide (1 mM) inhibited consumption of DCM by these degraders but not by transconjugants Methylobacterium extorquens AM1, expressing DCM dehalogenase but unable to grow on DCM. This indicates that the degrader strains possess energy-dependent systems of transport of DCM or chloride anions produced during DCM dehalogenation. Inducible proteins were found in the membrane fraction of A. methylovorans DM10 cells adapted to DCM and elevated NaCl concentration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|