Autor: L. A. Yakubov, T. I. Gainutdinov, M. I. Dobrikov, O. E. Shestova, Valentin V. Vlassov
Rok vydání: 2002
Předmět:
Zdroj: Russian Chemical Bulletin. 51:1198-1203
ISSN: 1066-5285
DOI: 10.1023/a:1020940210953
Popis: Interactions of double-stranded nucleic acids with cell surface proteins, which are involved in binding and transport of extracellular nucleic acids, were studied by the photoaffinity modification with a binary system of oligonucleotide conjugates. The photoreactive double-stranded complex involved an oligonucleotide template and two complementary to adjacent sequences oligonucleotide conjugates. One conjugate contained a photoreagent, viz., 4-azido-2,3,5,6-tetrafluorobenzaldehyde N-(3-aminopropionyl)hydrazone, at the terminus located in proximity to the terminus of another conjugate containing the sensitizer, viz., 9-aminomethylanthracene. Binding of photoreagent and the sensitizer to a single-stranded template yields the photoreactive center. Upon irradiation with visible light (400—580 nm), this photoreactive double-stranded complex forms covalent cross-linkages with oligonucleotide-binding surface proteins of eucaryotic SPEV cells.
Databáze: OpenAIRE