Detection of human papillomavirus in vulva cancer tissue
| Autor: | Tatyana A. Zykova, Konstantin Vladimirovich Dvadnenko, Natalya V Ohremenko, Ekaterina Alekseevna Nerodo, Anna V. Antonets, Galina Andreevna Nerodo |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Journal of Clinical Oncology. 31:e16514-e16514 |
| ISSN: | 1527-7755 0732-183X |
| Popis: | e16514 Background: Human Papillomavirus (HPV) status of the patients with vulva cancer was investigated retrospectively. Methods: We detected HPV of high cancerogenic risk (16, 18, 31, 33, 35, 45, 51, 52, 58, 59 types) in archival paraffin-embedded vulva cancer tissue of 58 patients aged from 26 to 82 years with I-IV grades. The extraction of DNA was performed with classical phenol-chloroform laboratory technic for further real-time PCR analysis (with HPV screening and HPV genotype kits of Amplisens). Results: HPV DNA was found out in 12 patients (20.6%). Among these patients 8 from 10 women (80%) belong to the group of reproductive age (26-50 years) whilst 4 from 48 (8.33%) were seniors. HPV of 16 type presented in 50%, HPV of 16 and 18 types – in 50% of all HPV positive patients of reproductive age. Among elderly patients 50 % had HPV of 16 type, 50 % - 16 and 31 types. Conclusions: Our investigations confirm the existence of two pathogenic variants of vulva cancer. For young age group there are up to 80% of HPV positive patients. The second elderly group (50-82 years) is not related with infection, there are only 8% of HPV positive patients. |
| Databáze: | OpenAIRE |
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