| Autor: |
Emily M. Eshleman, Taylor Rice, Crystal Potter, Amanda Waddell, Seika Hashimoto-Hill, Vivienne Woo, Sydney Field, Laura Engleman, Michael A. Schumacher, Mark R. Frey, Lee A. Denson, Fred D. Finkelman, Theresa Alenghat |
| Rok vydání: |
2023 |
| DOI: |
10.1101/2023.03.02.530792 |
| Popis: |
SUMMARYAllergy and anti-helminth immunity are driven by type 2 responses in mucosal tissues. Tuft cells are key regulators of type 2 immunity, however the factors that control these cells remain poorly understood. Here we find that butyrate-producing commensal bacteria decrease tuft cells in the intestine. Butyrate suppression of tuft cells required the epigenetic modifying enzyme histone deacetylase 3 (HDAC3), suggesting that HDAC3 may promote tuft cell-dependent immunity. Consistent with this, epithelial-intrinsic HDAC3 actively regulated tuft cell expansionin vivoand was required to induce type 2 immune responses during helminth infection. Interestingly, butyrate epigenetically restricted stem cell differentiation into tuft cells, and inhibition of HDAC3 in adult mice and human intestinal organoids was sufficient to block tuft cell expansion. Collectively, these data reveal an epigenetic pathway in stem cells that directs tuft cell differentiation, and highlight a new level of regulation through which commensal bacteria calibrate intestinal immunity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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