Chinese Medicine She-Xiang-Xin-Tong-Ning, Containing Moschus, Corydalis and Ginseng, Protects from Myocardial Ischemia Injury via Angiogenesis
| Autor: | Jin-xin Li, Xin-Yi Lan, Chun-Feng Zhang, Lu Gan, Xiao-Fan Zhang, Gui-yun Cao, Chao-lin Yang, Jia Li, Zhao-qing Meng |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Myocardial ischemia Chinese patent medicine biology business.industry Angiogenesis General Medicine Corydalis Traditional Chinese medicine 030204 cardiovascular system & hematology biology.organism_classification medicine.disease Coronary heart disease Angina 03 medical and health sciences Ginseng 0302 clinical medicine Complementary and alternative medicine 030220 oncology & carcinogenesis Internal medicine Cardiology Medicine business |
| Zdroj: | The American Journal of Chinese Medicine. 48:107-126 |
| ISSN: | 1793-6853 0192-415X |
| DOI: | 10.1142/s0192415x20500068 |
| Popis: | The Chinese patent medicine She-Xiang-Xin-Tong-Ning (SXXTN) is a clinical medication for coronary heart disease (CHD) and angina pectoris. This study aimed to investigate pharmacological effects of SXXTN and elucidate the role in angiogenesis on human umbilical vein endothelial cells (HUVECs) and acute myocardial ischemia (AMI) rats. We prepared SXXTN to treat the cells to reveal their effects on oxidative stress-damaged cell viability, as well as cell proliferation, migration, and tube formation processes. SXXTN was also used to treat coronary artery ligation-induced acute myocardial ischemia rats to confirm whether it had positive effect on myocardial issues by hematoxylin and eosin (HE), 2,3,5-triphenyltetrazolium chloride (TTC) staining and immunohistochemical staining. We measured the levels of peroxidative damage-related enzymes in cytoplasm and serum by biochemical kits and detected vascular endothelial growth factor (VEGF), angiotensin II (Ang II), thromboxane B2 (TXB2), and 6-keto-prostaglandin F1 alpha (6-keto-PGF1[Formula: see text]) levels in cells and rats by enzyme-linked immunosorbent assay (ELISA) kits. The results showed that SXXTN protects HUVECs against oxidative stress damage and reversed the decrease of superoxide dismutase (SOD), glutathione (GSH) and increase of creatine kinase (CK), lactate dehydrogenase (LDH) caused by oxidative stress. SXXTN promoted angiogenesis through stimulating cell migration, tube formation, and activating VEGF/VEGFR2 and ERK1/2 pathways. Furthermore, SXXTN reduced infarct size and inhibited PGI2/TXA2 imbalance, preventing atherosclerosis plaque rupture leading to worsening coronary heart disease. Taken together, we report the first in vivo and in vitro evidence that SXXTN reduced oxidative stress-mediated damage and enhanced angiogenesis, which might be useful in treatment of myocardial infarction. |
| Databáze: | OpenAIRE |
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