| Autor: |
Ai-ping Liu, Fang Wang, Fazheng Ren, Lu Jiang, Xing-Hua Guo |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Chinese Journal of Analytical Chemistry. 36:740-744 |
| ISSN: |
1872-2040 |
| DOI: |
10.1016/s1872-2040(08)60040-7 |
| Popis: |
High performance liquid chromatography (HPLC) was used to determine the antigenotoxic activity of Lactobacillus salivarius ( L. salivarius ) and was compared with the traditional SOS-chromotest assay. After co-incubation of L. salivarius FDB89 with 4-Nitroquinoline- N -oxide (4-NQO) (20 mg l −1 ) for 3 hours, SOS-chromotest assay was carried out to test the antigenotoxicity of L. salivarius FDB89. Simultaneously HPLC assay was used to determine the quantitative change of 4-NQO before and after co-incubation with L. salivarius FDB89. HPLC assay was performed on a reversed phase C18 column with UV detection at 365 nm and the flow rate was 1.0 ml min −1 . HPLC results clearly show that the toxic 4-NQO is converted to less toxic compounds and therefore its genotoxicity is inhibited. The genotoxicity measured by SOS-chromotest and HPLC methods were positively correlated, with a square correlation coefficient ( R 2 ) of 0.991. Under the above conditions, instrument detection limits were 1.2 ng. The recovery of 4-NQO was 85%–98% when the cell densities were in the range of 10 6 –10 9 cfu ml −1 . The results suggest that the HPLC method can be a useful alternative to SOS-chromotest assay in detecting the antigenotoxicity of lactobacillus, in being more sensitive and accurate than the latter. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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