Kinetics of ligand binding to nucleic acids at arbitrary occupancy

Autor:	S. Yu. Babayan, V. I. Tairyan, Poghos O. Vardevanyan, H. V. Arakelyan, Marine A. Parsadanyan, V.B. Arakelyan
Rok vydání:	2006
Předmět:	Ligand efficiency Reaction rate constant Computational chemistry Chemistry Stereochemistry Kinetics Biophysics Nucleic acid Sorption isotherm Ligand (biochemistry) Probabilistic description Dissociation (chemistry)
Zdroj:	Biophysics. 51:370-375
ISSN:	1555-6654 0006-3509
Popis:	Ligand binding to nucleic acids is described in the context of the theory of random processes. It is shown that a probabilistic description of the binding of a ligand to a nucleic acid yields the kinetics of the number of bound ligands at arbitrary occupancy, as well as the steady-state value and the variance. It is demonstrated that a combined analysis of the adsorption isotherm and the kinetics of ligand binding to nucleic acids makes it possible to determine the rate constants for association and dissociation of the ligand-nucleic acid complex. The results obtained are compared with the case of low occupancy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1e2336d2f1977e3438f4f7e91930db97 https://doi.org/10.1134/s0006350906030067 Zobrazit plný text záznamu Full text from SpringerLink