Protease-mediated prophenoloxidase activation in the haemolymph of American cockroach Periplaneta americana
Autor: | T Thangaraj, P Manikandan, M. Aruchami, M. Rabeeth |
---|---|
Rok vydání: | 1995 |
Předmět: |
animal structures
Chymotrypsin Protease biology Kunitz STI protease inhibitor Physiology medicine.medical_treatment fungi Subtilisin Prophenoloxidase Trypsin Biochemistry Molecular biology Benzamidine chemistry.chemical_compound chemistry Thermolysin biology.protein medicine Molecular Biology medicine.drug |
Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 111:607-613 |
ISSN: | 1096-4959 |
Popis: | Prophenoloxidase has been successfully obtained from the haemolymph of the cockroach Periplaneta americana using cane sugar saline solution. The proenzyme was activated by various exogenously added proteases such as chymotrypsin, trypsin, subtilisin and thermolysin. Thermolysin was found to be the greatest activator, followed by chymotrypsin and subtilisin. Chymotrypsin activation showed a lag period when compared with the other proteases tested, indicating that activation by chymotrypsin followed an indirect path, whereas, subtilisin and thermolysin activated the proenzyme directly. Exogenously added protease inhibitor showed inhibition towards protease-mediated prophenoloxidase activation. Benzamidine inhibited chymotrypsin and trypsin activation, whereas soybean trypsin inhibitor inhibited trypsin. In situ inhibitor isolated from the haemocytes of Periplaneta americana inhibited the prophenoloxidase activation and showed evidence for the presence of a built-in inhibition system for the release of the components of the prophenoloxidase activating system of P. americana . Electrophoretic localization of activated phenoloxidase showed two bands, suggesting the dimeric condition of high mol. wt prophenoloxidase. |
Databáze: | OpenAIRE |
Externí odkaz: |