High concentrationtrans form unsaturated lipids detected in a HeLa cell by Raman microspectroscopy

Autor:	Hiro-o Hamaguchi, Chikao Onogi, Michiyo Motoyama
Rok vydání:	2008
Předmět:	High concentration In situ biology Chemistry Cell Analytical chemistry Living cell Intensity ratio biology.organism_classification Raman microspectroscopy HeLa symbols.namesake medicine.anatomical_structure medicine symbols General Materials Science Raman spectroscopy Spectroscopy
Zdroj:	Journal of Raman Spectroscopy. 39:555-556
ISSN:	1097-4555 0377-0486
DOI:	10.1002/jrs.1900
Popis:	High concentration trans form unsaturated lipids have been found in a HeLa cell by Raman microspectroscopy. Two CC stretch bands are observed simultaneously at 1669 cm−1 (trans form) and at 1656 cm−1 (cis form) in a Raman spectrum obtained from a small area (1 µm in diameter) in a HeLa cell. The intensity ratio 1669/1656 indicates that the concentration of the trans form is as high as that of the cis. It is demonstrated that Raman microspectroscopy provides a powerful and unique means for in situ and noninvasive structural characterization of unsaturated lipids in a living cell. Copyright © 2008 John Wiley & Sons, Ltd.
Databáze:	OpenAIRE
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