THE NUTRITION OF ANIMAL TISSUES CULTIVATED IN VITRO

Autor:	Helen J. Morton, Joseph F. Morgan
Rok vydání:	1957
Předmět:	chemistry.chemical_classification Methionine Arginine Cell Biology Biology Amino acid Glutamine chemistry.chemical_compound chemistry Biochemistry Valine Leucine Isoleucine Amino acid synthesis
Zdroj:	The Journal of Biophysical and Biochemical Cytology. 3:141-150
ISSN:	1540-8140 0021-9525
DOI:	10.1083/jcb.3.2.141
Popis:	1. The amino acid requirements of freshly explanted chick embryonic heart tissues cultivated in completely synthetic media have been determined, employing a nutritional depletion technique. Arginine, histidine, lysine, tyrosine, tryptophan, phenylalanine, cystine, methionine, threonine, leucine, and valine were found to be essential. Serine, isoleucine, glycine, and glutamine were found to be non-essential. Glutamic acid, aspartic acid, α-alanine, proline, and hydroxyproline were found to be inhibitory in this test system. 2. A total amino acid level of approximately 100 mg. per cent was found to be optimal and DL-amino acids were found to be non-toxic, unless used in high concentrations. 3. A comparison has been made of the amino acid requirements of various types of tissue cultures, of the chick, and of man and certain differences in these requirements have been discussed.
Databáze:	OpenAIRE
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