Popis: |
Hemocytes of healthy (untreated) and Beauveria bassiana (Bals.-Criv.) Vuill.-treated nymphs and adults Periplaneta americana (L.) (Blattodea: Ectobiidae) were examined with phase contrast microscopy. The total hemocyte counts (THCs) and differential hemocyte counts (DHCs) were estimated and compared between the untreated and treated nymphs and adults; significant augmentation of THC was observed in the treated 3rd, 4th, 5th instars and adults at 6 h after treatment (HAT), compared to the untreated individuals, followed by its suppression. In addition to changes in the population dynamics of prohemocytes (PRs), plasmatocytes (PLs), granulocytes (GRs) and spherulocytes (SPs), their morphology was also affected. GRs, predominant hemocytes involved in cell-mediated defense, responded to the infection differently including the formation of fine pseudopodia–like cytoplasmic extensions and, they clumped together. |