| Autor: |
R. J. Condron, Alan G. Marshall |
| Rok vydání: |
1985 |
| Předmět: |
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| Zdroj: |
Journal of Microscopy. 140:109-118 |
| ISSN: |
0022-2720 |
| DOI: |
10.1111/j.1365-2818.1985.tb02666.x |
| Popis: |
SUMMARY In coated frozen-hydrated gelatin gels the backscattered electron yield does not increase during electron irradiation as it does in uncoated samples. Neither is the backscattered electron yield greater from coated frozen-hydrated gels than that from more conductive organic samples. This is interpreted as indicating that a significant distortion of the electron interaction volume, due to the development of a space charge, does not occur in electron irradiated frozen-hydrated gelatine gels when they are coated with a conducting coat. The depth resolution as estimated from models of biological samples in the form of frozen-hydrated photographic film and frozen-hydrated sections of gelatine gel is consistent with that computed from X-ray depth distribution curves, i.e. close to 2.0 μm at 15 kV. Lateral resolution was estimated from photographic film to be close to 2.0 μm also, at 15 kV. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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