Evaluation of cytotoxicity, genotoxicity, and zebrafish embryo toxicity of mixtures containing Hyssopus officinalis, Morus alba, Engraulis japonicus, and 27 other extracts for cosmetic safety assessment
| Autor: | Yuna Ha, Hee Kyung Jeon, Youn-Jung Kim, Ju-Young Ko, Inchul Hwang, Jeongyeon Choi, Young-Jin Kim |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
animal structures biology Chemistry Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Toxicology biology.organism_classification medicine.disease_cause Pathology and Forensic Medicine Comet assay 03 medical and health sciences HaCaT 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Toxicity medicine Viability assay Food science General Pharmacology Toxicology and Pharmaceutics Cytotoxicity Zebrafish Hyssopus officinalis Genotoxicity |
| Zdroj: | Molecular & Cellular Toxicology. 17:221-232 |
| ISSN: | 2092-8467 1738-642X |
| DOI: | 10.1007/s13273-021-00128-7 |
| Popis: | Although various natural ingredients and formulated reagents are used as ingredients in cosmetics, it is important to have alternatives to animal tests, such as in vitro tests of toxicity, as animal testing of cosmetics is prohibited in many countries. Here, cytotoxicity, genotoxicity, and embryotoxicity on zebrafish embryos were evaluated to confirm the safety of ingredients formulated with 30 extracts from natural resources used as cosmetic ingredients. The blended ingredients were tested by dividing them into mixtures of 25 natural products (NMA), 30 natural products (NMB), and concentrated NMB (NMC), depending on the number of blended natural components and their application. First, in vitro, HaCaT cells were treated with concentrations of 0 to 20% for 24 h to measure cell viability. DNA damage within the IC20 value was measured by comet assay. In the comet assay, 10 mM H2O2 was used as a positive control. Additionally, zebrafish embryos were used as a substitute for conventional animal testing to observe natural mixture toxicity. The olive tail moment (OTM) were not affected by NMA, NMB, or NMC in comparison with the positive control versus three representatives within the IC20 value concentration of each mixture. Zebrafish embryo results were analysed in a manner similar to the cytotoxicity test results. NMA mixtures showed no cytotoxicity, genotoxicity, or toxicity on zebrafish embryos. Other mixtures showed non-toxicity within the representatively concentrated range. Therefore, the 3 mixtures containing Hyssopus officinalis, Morus alba, Engraulis japonicus, and 27 other extracts were considered to be not significantly toxic in HaCaT cell and zebrafish, so it is suggested that they could be used as cosmetic ingredients. |
| Databáze: | OpenAIRE |
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