Evaluation of Xylose-Utilising Yeasts for Xylitol Production from Second-Generation Ethanol Vinasse and Effect of Agitation Intensity in Flask-Scale Xylitol Production
| Autor: | Sreyden Hor, Mallika Boonmee Kongkeitkajorn, Alissara Reungsang |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Sains Malaysiana. 52:175-185 |
| ISSN: | 2735-0118 0126-6039 |
| DOI: | 10.17576/jsm-2023-5201-14 |
| Popis: | This study aimed to select a yeast strain that effectively utilises xylose to produce xylitol from the vinasse of ethanol broth obtained from the fermentation of sugarcane bagasse hydrolysate. Eleven strains of xylose-fermenting yeasts were evaluated for their abilities to utilise xylose and produce xylitol. Two strains that showed outstanding performance in the semi-defined xylose medium were selected for further testing with a vinasse medium. Candida guilliermondii TISTR 5068 showed a superior xylitol production of 7.03 ± 0.08 g/L with the xylitol yield of 0.70 g/gxylose when cultured in bagasse-based ethanol vinasse. The strain was further tested for its xylitol production performance when cultured at four different agitation intensities. Excessive agitation resulted in a rapid xylitol production rate but caused xylitol consumption once the xylose was depleted. Moderate agitation resulted in the highest xylitol yield of 0.79 g/gxylose. The results of this study have provided important information for the development of the xylitol production process using waste streams from cellulosic ethanol production. |
| Databáze: | OpenAIRE |
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