One-step detection of ochratoxin A in cereal by dot immunoassay using a nanobody-alkaline phosphatase fusion protein
| Autor: | Xuerou Wang, Jingwen Lv, Zongwen Tang, Xing Liu, Xiangrong Hu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Ochratoxin A
Reproducibility Chromatography medicine.diagnostic_test Chemistry 010401 analytical chemistry food and beverages 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Fusion protein 0104 chemical sciences chemistry.chemical_compound Immunoassay medicine Alkaline phosphatase Sample preparation Naked eye 0210 nano-technology Ochratoxin Food Science Biotechnology |
| Zdroj: | Food Control. 92:430-436 |
| ISSN: | 0956-7135 |
| DOI: | 10.1016/j.foodcont.2018.05.013 |
| Popis: | In this work, a polyvinylidene fluoride (PVDF) membrane-based dot immunoassay was developed using a nanobody-alkaline phosphatase (Nb-AP) fusion protein for one-step, qualitative, and visual detection of ochratoxin A in cereals. Using the optimal assay conditions, the assay was completed in 6 min and the cut-off limit of this method, assessed by the naked eye, was 5 μg/kg for ochratoxin A. This method was designed to be simple, fast, and low cost without time-consuming sample preparation and cleanup procedures. Good accuracy and reproducibility were obtained in a spike-and-recovery experiment. Sample analysis results of the Nb-AP-based dot immunoassay and LC-MS/MS were in good agreement with each other. These results demonstrated that the Nb-AP-based dot immunoassay could be an acceptable method for one-step and visual detection of ochratoxin A in cereal without the need for special instruments. |
| Databáze: | OpenAIRE |
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