Determination of varenicline after photochemical fluorescence enhancement using spectrofluorimetry and high-performance liquid chromatography
| Autor: | Ricardo Q. Aucélio, Sarzamin Khan, Carlos A.T. Toloza, Alessandra L. M. C. da Cunha, Ana C. P. Osorio, Joseany M.S. Almeida |
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| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Analyte 010401 analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences Fluorescence High-performance liquid chromatography Orders of magnitude (mass) 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Linear range Methanol 0210 nano-technology Derivatization Spectroscopy |
| Zdroj: | Microchemical Journal. 144:172-179 |
| ISSN: | 0026-265X |
| Popis: | Photochemical derivatization (at 253 nm) of varenicline in alkaline medium (in NaOH 0.64 mol L−1) was successfully achieved aiming to induce strong fluorescence for quantitative purposes. The intense fluorescence from the produced photo-derivative covered a linear range of three orders of magnitude (10−8–10−6 mol L−1 range) allowing the spectrofluorimetric determination of the analyte in pharmaceutical formulations. For urine, the UV exposed sample was analyzed by high-performance liquid chromatography using borate buffer (0.01 mol L−1, pH 10.0). Separations were made using a C18 column with borate buffer (0.01 mol L−1, pH 10)/methanol (30:70% v/v) in isocratic elution. Detection limit was 2.5 × 10−7 mol L−1 for determination using HPLC. Measurement uncertainty was estimated and both methods were applied for the determination of varenicline in a pharmaceutical preparation and urine samples with acceptable recoveries using no expensive and toxic derivatization dyes. |
| Databáze: | OpenAIRE |
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