| Autor: |
Guy Dodson, Keith S. Wilson, S. V. Pletnev, Tatyana V. Demidkina, Alfred A. Antson, E. G. Harutyunyan |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Biochemistry of Vitamin B6 and PQQ ISBN: 9783034873956 |
| DOI: |
10.1007/978-3-0348-7393-2_30 |
| Popis: |
Monovalent cation binding site of the enzyme was determined using X-ray data obtained from apoenzyme crystals soaked in K+ and Cs+ containing solutions. Glu 69 is involved in formation of that site and is conserved in all other tyrosine phenol-lyases and tryptophan indol-lyases with known sequence. Three dimensional structures of holoenzyme and its complex with 3-(4-hydroxyphenyl)propionic acid were solved and refined at 2.7A and 2.5 A respectively. The structures explicitly reveal the cofactor and substrate binding pockets. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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