Variability in microsatellite DNA markers in gynogenetic and backcross progenies obtained from ornamental (koi) carp (Cyprinus carpioL.) × goldfish (Carassius auratusL.) hybrid females
| Autor: | Kyle J. Schneider, Ammu Anil, Boris Gomelsky |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Genetics biology 010604 marine biology & hydrobiology fungi 04 agricultural and veterinary sciences Aquatic Science biology.organism_classification 01 natural sciences Cyprinus Genetic marker Genotype Backcrossing 040102 fisheries 0401 agriculture forestry and fisheries Microsatellite Ploidy Carp Hybrid |
| Zdroj: | Aquaculture Research. 47:1150-1155 |
| ISSN: | 1355-557X |
| DOI: | 10.1111/are.12570 |
| Popis: | Inheritance and segregation at five microsatellite loci were studied in diploid gynogenetic and triploid backcross progenies obtained from koi × goldfish hybrid females, which produce diploid eggs. Gynogenetic and backcross progenies were obtained from three individual hybrid females by inseminating eggs with genetically inactivated and intact sperm of parental species respectively; no shock treatments were applied to the early embryos. Complete absence of paternally specific alleles at all investigated microsatellite loci has proven successful genetic inactivation of spermatozoa by irradiation and confirmed gynogenetic origin of progenies. Genotypic segregations at microsatellite loci showed almost complete homogeneity of gynogenetic progenies and their identity to female parents. These results correspond with previous cytogenetic data on the occurrence of premeiotic endomitosis in hybrid females producing diploid eggs. Fish from triploid backcross progenies had genotypes resulting from combination of entire diploid female genome and haploid genome from male. |
| Databáze: | OpenAIRE |
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