Impact of oral probiotic treatment in murine model of atopic dermatitis
| Autor: | Ariee Jung, Julie A Swartzendruber, Ryan Incrocci, Rosalind Del Toro |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 206:94.19-94.19 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.206.supp.94.19 |
| Popis: | The number of people with allergies has been on the rise over the last half-century; and the most common allergen afflicting humans, dogs and cats is the house dust mite (HDM). The rise in allergic disease correlates with an increasingly sterile living environment and reduced exposure to bacteria. We predict that exposure to non-pathogenic bacteria helps educate our immune system to become tolerant to innocuous material, such as allergens. Our previous studies have found that Bacillus subtilis, a gram-positive spore forming bacterium, can reduce the symptoms of HDM-induced allergic lung disease. Our current work is aimed at determining if B. subtilis can also provide protection in a murine model of atopic dermatitis. To test this, we administer B. subtilis by oral gavage 3 days before allergen exposure and weekly-post allergen exposure (total of 9 applications), while HDM extract in PBS is applied topically, after shaving the mouse back, 3 times a week for 8 weeks (total of 24 applications). After 8 weeks the serum is assessed for HDM-specific antibodies by ELISA. Additionally, we monitor symptoms of atopic dermatitis: reduced scratching, skin lesions and histological thickening of the skin. Our results, have identified HDM-specific IgM, IgG1 and IgE levels are unaffected by oral gavage of B. subtilis. This suggests orally administered probiotics are not sufficient to protect from topical allergen sensitization |
| Databáze: | OpenAIRE |
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