Resistance against Fusarium Head Blight in Transgenic Wheat Plants Expressing theScNPR1gene
| Autor: | Hongxiang Ma, MiaoPing Zhou, Guihong Yu, Jingbao Yao, Xu Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fusarium Secale Genetics biology Physiology Transgene food and beverages Plant Science Genetically modified crops Plant disease resistance biology.organism_classification 01 natural sciences 03 medical and health sciences 030104 developmental biology Agronomy Agronomy and Crop Science Gene Systemic acquired resistance 010606 plant biology & botany Regulator gene |
| Zdroj: | Journal of Phytopathology. 165:223-231 |
| ISSN: | 0931-1785 |
| DOI: | 10.1111/jph.12553 |
| Popis: | Fusarium head blight (FHB) is a severe global wheat disease that may cause severe yield losses, especially during epidemic years. Transforming the regulatory genes in the metabolic pathways of disease resistance into wheat via transgenic methods is one way to improve resistance to FHB. ScNPR1 (Secale cereale-NPR1), a regulatory gene for systemic acquired resistance (SAR), was isolated from S. cereale cv Jingzhouheimai and transformed into the moderately FHB-susceptible wheat variety Ningmai 13. RT-PCR analysis indicated that the ScNPR1 gene was stably expressed in transgenic plants. An evaluation of the resistance to FHB revealed that six ScNPR1 transgenic lines (NP1, NP2, NP3, NP4, NP5 and NP6) exhibited significantly higher FHB resistance than the wild-type wheat Ningmai 13 and the null-segregated plants. The expression of pathogenesis-related (PR) genes after Fusarium graminearum inoculation was earlier or higher than those in the wild-type variety Ningmai 13. The high expression in the early stages of PR genes should account for the enhanced FHB resistance in the transgenic lines. Our results suggest that overexpression of ScNPR1 could be used to improve FHB resistance in wheat. |
| Databáze: | OpenAIRE |
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