Expression and Characterization of Rice-produced Recombinant Porcine Lactoferrin and its Antioxidant Activities
| Autor: | Ray-Bin Chen, Chang-Sheng Wang, Kun-Ting Hsieh, Wei-Chih Lin, Kuan-Chih Lee, Tzu-Tai Lee, Liang-Jwu Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant medicine.medical_treatment Biomedical Engineering Biophysics Bioengineering Peptide 01 natural sciences Biochemistry law.invention 03 medical and health sciences N-linked glycosylation Western blot law medicine chemistry.chemical_classification biology medicine.diagnostic_test Lactoferrin Chemistry Molecular biology Genetically modified rice 030104 developmental biology biology.protein Recombinant DNA 010606 plant biology & botany Biotechnology |
| Zdroj: | The Open Biotechnology Journal. 14:94-106 |
| ISSN: | 1874-0707 |
| Popis: | Background: Lactoferrin (LF) exhibits multiple beneficial biological activities and thus has been used as a health food and additive. To broaden its application in the food industry, the porcine LF (pLF) gene has been engineered into rice to produce recombinant LF (rpLF) for use as a food additive. The iron-binding and antimicrobial activities of rpLF and its positive effects on early weaned piglets have been previously evaluated, yet several features, such as the signal peptide removal, glycosylation sites and antioxidant activity of rpLF, have not been fully characterized. Objective: In this work, the rice-produced rpLF was purified and its biochemical structure and antioxidant activities characterized. Methods: HPLC, Western blot, PAS/VVL/PNA staining, Edman degradation assay, MALDI-TOF, LC-MS/MS and antioxidant activity assays were performed. Results: The results showed that this purified rpLF is a mature form of LF; its signal peptide was correctly removed, and two N-glycosylation sites located at N365 and N472 were identified. The molecular mass heterogeneity of rpLF could be eliminated by treatment with PNGase glycosidase, suggesting that different degrees of N-glycosylation occur in rpLF. A series of assays including the iron chelating activity, reducing power assay, lipid peroxidase activity and radical-scavenging activity showed that the antioxidant activity of rice-produced rpLF was equivalent to that of bovine LF. Conclusion: Rice-produced rpLF was correctly processed post-translationally and displayed antioxidant activity equivalent to that of bovine LF; thus, rice-produced rpLF can be recognized as a plant-based antioxidant to be used as a functional additive in animal feed and for the food industry. |
| Databáze: | OpenAIRE |
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