Isolation of tissue-specific inhibitors of DNA synthesis (chalones) from rat liver

Autor:	Ketlinskiĭ Sa, Parfenova Ev
Rok vydání:	1983
Předmět:	Kidney Ethanol DNA synthesis Size-exclusion chromatography Biological activity General Medicine Biology General Biochemistry Genetics and Molecular Biology Liver regeneration In vitro chemistry.chemical_compound medicine.anatomical_structure chemistry Biochemistry medicine DNA
Zdroj:	Bulletin of Experimental Biology and Medicine. 96:926-929
ISSN:	1573-8221 0007-4888
Popis:	Two fractions of non-cytotoxic and tissue-specific inhibitors of 3H-thymidine incorporation into DNA of rat liver regenerating after partial hepatectomy in vitro were obtained by gel filtration on a Sephadex-75 column of the ethanol precipitated aqueous extract. One of these fractions (Ve/Vo = = 2.0; Kav = 0.4) was found to be heterogenous and enriched with proteins with a molecular weight of 17 000-30 000 D. It was biologically active at concentrations exceeding 0.25 mg/ml. The biological activity of the other fraction (Ve/Vo = 3.0; Kav = 1) occurred at concentrations under 0.005 mg/ml. The main component of this fraction was discovered to be a protein with a molecular weight of 17 000 D.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1d6a92dbd499575a1d104c8de2ce5b35 https://doi.org/10.1007/bf00833039 Zobrazit plný text záznamu Full text from SpringerLink