Molecular characterization and virulence of Beauveria bassiana and Metarhizium anisopliae against Galleria mellonella (Lepidoptera: Pyralidae) and Tenebrio molitor (Coleoptera: Tenebrionidae) larvae
| Autor: | Margy Alejandra Esparza Mora, Janaina Ribeiro Costa Rouws, Julie G. Chacón-Orozco, Marcelo Elias Fraga, Ricardo Harakava |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
biology Beauveria bassiana Metarhizium anisopliae Virulence 04 agricultural and veterinary sciences Plant Science Bassiana biology.organism_classification 01 natural sciences Microbiology Conidium Lepidoptera genitalia Galleria mellonella 010602 entomology Infectious Diseases 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Pyralidae |
| Zdroj: | African Journal of Microbiology Research. 10:662-668 |
| ISSN: | 1996-0808 |
| Popis: | Entomopathogenic fungi considerably vary in their action and virulence mode. The contamination mainly depends on the adhesion and penetration of the fungus in the host integument. Four isolates from Beauveria bassiana and Metarhizium anisopliae were molecularly characterized by rDNA-ITS sequencing. Their virulence against last instar larvae of Galleria mellonella and Tenebrio molitor at four conidia concentrations: 1×106, 1×107, 1×108 and 1×109 conidia.ml-1 were in vitro evaluated and the lethal concentrations (LC50 and LC90) as lethal time (LT50 and LT90) were determined. Sequencing of rDNA-ITS 500 bp fragments allowed the identification of Ma10MI, Ma12MI, Ma58MI and Ma11MI isolates as M. anisopliae and Bb11MI, Bb79MI, Bb53MI and Bb27MI isolates as B. bassiana by sequence comparison to GenBank. According to the pathogenicity test, B. bassiana strains with higher performance for G. mellonella were Bb53MI and Bb79MI with LC50= 3.98×107 and 1.04×107 conidia.ml-1 and LT50= 5.46 and 5.27 days, respectively, as well as to T. molitor was Bb79MI with LC50= 1.03×107 conidia.ml-1 and LT50= 5.57 days. The M. anisopliae strains with the best performance were Ma58MI and Ma10MI both T. molitor (LC50= 1.0×106 and 1.0×107 conidia.ml-1 and LT50= 4.06 and 5.22 days, respectively) and G. mellonella (LC50= 6.3×107 and 1.1×108 conidia.ml-1 and LT50= 5.18 and 6.37 days, respectively), these isolates might be considered as new promising candidates for the microbial pest control. Key words: Pathogenicity, molecular taxonomy, entomopathogenic fungi, microbial control. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|