Cell viability and bacterial reduction activity of Ag-modified bentonite
| Autor: | Kathrina Lois M. Taaca, Eleanor M. Olegario, Luigi A. Dahonog |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
010302 applied physics
Biocompatibility Chemistry 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Mordenite Silver nitrate chemistry.chemical_compound Adsorption 0103 physical sciences Bentonite Hectorite 0210 nano-technology Antibacterial activity Clay minerals Nuclear chemistry |
| Zdroj: | Materials Today: Proceedings. 16:1782-1788 |
| ISSN: | 2214-7853 |
| Popis: | The interest on the potential biomedical applications of clay materials are recently growing. Among the clay minerals, it was found that the smectite group clays are the most suitable for adsorption processes. This group includes bentonite in which the structure is composed of octahedral and tetrahedral layers of silica. In this study, bentonite clays from SAILE Industries, Inc. in Mangatarem, Pangasinan, Philippines were characterized and fabricated as Ag-bentonite composites to determine the potential of the mineral for biomedical applications. Bentonite clay powder was mixed with silver nitrate solution via ion-exchange process. The synthesized powders were filtered using centrifugation and washed with deionized water to remove any excess silver ions. Drying followed at 60°C and stored in a dark container prior to characterization. XRD and XRF was used to evaluate the phase composition and chemical analysis of the Ag-bentonite composite, respectively. The biological tests were also done to investigate the antibacterial and cell viability of the sample.XRD result showed that the sample is composed of the montmorrilonite, hectorite and mordenite phases. Chemical analysis revealed the presence of Ag in the bentonite structure with a content of about 14.24±0.13%. Antibacterial activity of the sample was evaluated using plate reduction method where about 99% reduction of S. Aureus was observed after 24 h. Moreover, approximately 89±4.37% live cells was detected after the 24-h incubation of the sample using lymphocyte culture. With these results, the synthesized AgB samples may find suitable future biomedical use due to its stable structure, antibacterial and biocompatibility properties |
| Databáze: | OpenAIRE |
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