| Autor: |
Imtiaz Gilliani, Hiam Chemaitelly, AlMaslamani Mars, Molawi Nha, Roberto Bertollini, Peter Coyle, Morris Bd, Rahim Hfa, Nasser Al-Ansari, Patrick Tang, Andrew Jeremijenko, Ali Nizar Latif, Anvar Hassan Kaleeckal, Einas Al Kuwari, Kahlout Rae, Raddad Lja, Saeb H, Thani Sma, Kacem Mabh, A. L. Al Khal, Kaabi Sa |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
IntroductionThe Cycle Threshold (CT) value in Real-time Polymerase Chain Reaction (RT-PCR) is where a target specific amplification signal becomes detectable and can infer viral load, risk of transmission and recovery in SARS-CoV-2 infections. Adoption into routine practice is however uncommon.Gap StatementThe lack of inclusion of CT values when reporting SARS-CoV-2 RT-PCR results in routine practice.AimTo use CT values when reporting SARS-CoV-2 RT-PCR results in Qatar to aid clinical interpretation and patient management.MethodologyRoutine CT values across 3 different RT-PCR platforms were reviewed for concordance at presentation and clearance in patients with COVID-19. An Indicative Threshold of CT 30 based on viral clearance kinetics categorized low and high CT values.ResultsThere was very high Correlation and Kappa Score agreement between the different gene targets in each platform (pConclusionIncorporation of CT values into routine practice is possible across different RT-PCR platforms and adds useful information for patient management. The use of an Indicative Threshold and interpretive comments improves clinical interpretation of the result and could be a model for reporting other respiratory infections. The current accepted practice of withholding CT values should be reviewed by the profession, accreditation bodies and regulators. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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