The role of pigmentation in the growth and survival of the methanol-utilising bacterium, Pseudomonas extorquern
| Autor: | Christopher D. B. Scott, Stephen G. Oliver, David E. F. Harrison, Hamida Merabtine |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Journal of Chemical Technology and Biotechnology. 32:1059-1064 |
| ISSN: | 1935-181X 0142-0356 |
| DOI: | 10.1002/jctb.5030320751 |
| Popis: | The role of pigmentation in the growth and survival of Pseudomonas extorquens (NCIB 9399) has been examined in both the pink wild-type strain and a yellow mutant (T358) derived from it. The effect of inhibitors of carotenoid biosynthesis has also been assessed. The yellow mutant was found to compete out the wild-type when the two organisms were grown together in methanol-limited continuous culture. The methanol dehydrogenase of the mutant was found to have a lower Km and higher Vmax than that of the wild-type. The response of mutant and wild-type cells to the growth inhibitory effects of methanol was similar. Moreover, incubation with chemical inhibitors of carotenoid biosynthesis did not alter the growth rate of the wild-type over a range of methanol concentrations. The pink pigment did, however, have a photoprotective effect and stationary phase wild-type cells were more resistant to the killing effects of ultraviolet light than the yellow mutant cells. |
| Databáze: | OpenAIRE |
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