Electrophoretic detection of alginate by Hematoxylin and Eosin fluorescence: Implications in cell encapsulation/tissue engineering
| Autor: | Gurudutta Gangenahalli, Yogesh Verma, Rajendra Prashad Tripathi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Chromatography
Polymers and Plastics Chemistry General Chemical Engineering H&E stain 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Fluorescence Stain 0104 chemical sciences Rhodamine chemistry.chemical_compound Electrophoresis Agarose gel electrophoresis Materials Chemistry Environmental Chemistry Agarose 0210 nano-technology Cell encapsulation |
| Zdroj: | Reactive and Functional Polymers. 102:130-136 |
| ISSN: | 1381-5148 |
| DOI: | 10.1016/j.reactfunctpolym.2016.03.016 |
| Popis: | Alginate is a biocompatible polymer commonly used for cell encapsulation and synthesis of biomaterials in pure or modified form. In this study we have developed a fluorescence based method to visualize Hematoxylin and Eosin (H&E) stained alginate (pure/protein modified) by electrophoresing it in agarose gel, followed by its UV exposure. The shift in the migration pattern of H&E stain indicates presence of alginate, whereas the conjugated protein (fibronectin) could be detected by UV spectroscopy. H&E stain was found to specifically and permanently stain alginate and the fluorescence and migration pattern of H&E was not affected in the presence of other dye (Rhodamine β Isothiocyanate - RBITC) conjugated to alginate. We found that the presence of all the ingredients of H&E is mandatory to influence the migration pattern of this dye (bound to alginate) upon agarose gel electrophoresis. The main advantage of this method is that it is cheaper and faster than the other methods for alginate visualization and can be accomplished with standard lab chemicals and equipment. |
| Databáze: | OpenAIRE |
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