Myrtol standardized affects mucociliary clearance
| Autor: | Shi Li, Jing Liu, Siew Shuen Chao, Yingying Li, Noam A. Cohen, Feng Gang Yu, Somasundaram Subramaniam, Chun Wei Li, De Yun Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Goblet cell Pathology medicine.medical_specialty business.industry Mucociliary clearance Cilium Cellular differentiation respiratory system 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Otorhinolaryngology Ciliogenesis Immunology and Allergy Medicine Progenitor cell 030223 otorhinolaryngology business Nasal symptoms Progenitor |
| Zdroj: | International Forum of Allergy & Rhinology. 7:304-311 |
| ISSN: | 2042-6976 |
| Popis: | Background Myrtol standardized (Gelomyrtol forte) has been shown to be effective in controlling nasal symptoms of rhinosinusitis by promoting mucociliary clearance. Our aim was to evaluate the short- and long-term effects of myrtol on ciliated columnar cells and goblet cells in an in-vitro setting. Methods Nasal epithelial cells were harvested (42 days) from an air-liquid interface (ALI) culture of human nasal epithelial stem/progenitor cells (hNESPCs), which was derived from biopsies of nasal inferior turbinate mucosa. Myrtol 0.1% was applied to the ALI culture system at 2 different time-points (day 0 and day 35) on progenitor and differentiated cells. Ciliary beat frequency (CBF), supernatant fluid, and ciliated and goblet cell markers were evaluated after short- (7 days) and long-term (42 days) treatment. Results In the long-term treatment with myrtol, there was an increase in cilia area (type IV β-tubulin+, 1.53-fold, p = 0.031) and ciliogenesis-related markers (Foxj1 and CP110) with no change in CBF, as compared with control. In addition, the short-term myrtol treatment group exhibited greater mucin secretion compared with control. Conclusion This study demonstrates, through cellular and molecular mechanisms, that myrtol standardized enhances the mucus production from goblet cells in the short term, and promotes ciliated cell differentiation in the long term. |
| Databáze: | OpenAIRE |
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