Analysis of intraspecific nuclear DNA content variation in Gleditsiatriacanthos by flow cytometry

Autor:	C. M. Wake, L. K. Jost, P. R. Schaefer, D. P. Evenson
Rok vydání:	1995
Předmět:	Global and Planetary Change Ecology medicine.diagnostic_test Range (biology) fungi Population genetics Forestry Edaphic Biology Intraspecific competition Nuclear DNA Flow cytometry Salinity Variation (linguistics) Botany medicine
Zdroj:	Canadian Journal of Forest Research. 25:440-445
ISSN:	1208-6037 0045-5067
DOI:	10.1139/x95-049
Popis:	Honeylocust trees (Gleditsiatriacanthos L.) are distributed across a wide range of climatic and edaphic conditions, demonstrating considerable variation in cold, drought, and salinity tolerances. In many angiosperm species, an evolutionary change in nuclear DNA content accompanies colonization into more stressful environments. Fresh leaf nuclei were isolated from 22 honeylocust sources from across the species' native range, mixed with trout red blood cells, stained with propidium iodide, and their DNA content was measured by flow cytometry. According to DNA stainability of the trout red blood cells, leaf nuclei had a DNA content of 1.72 ± 0.02 pg (mean ± SD). Variation in DNA content among the sources was not significant (p
Databáze:	OpenAIRE
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