Expression of Pseudorabies Virus (PRV) Glycoproteins gB, gC and gD using Bacterial Expression System
| Autor: | Bit-Na-Rae Yun, Soo-Dong Woo, Sung Min Bae, Jun Beom Lee, Hee Jung Kim |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
medicine.diagnostic_test biology lac operon Pseudorabies biology.organism_classification Virology Molecular biology Herpesvirus glycoprotein B Virus law.invention chemistry.chemical_compound chemistry Western blot law medicine Recombinant DNA Sodium dodecyl sulfate Glycoprotein |
| Zdroj: | International Journal of Industrial Entomology. 23:147-153 |
| ISSN: | 1598-3579 |
| DOI: | 10.7852/ijie.2011.23.1.147 |
| Popis: | The Pseudorabies (PR), also called Aujeszky`s disease (AD), is an infectious viral disease caused by an alpha herpes virus and has domestic and wild pigs, as well as a wide range of domestic and wild animals, as the natural host. Pseudorabies virus (PRV) virions contain several envelope glycoproteins. Among them, gB, gC and gD are regarded as the major immunogenic proteins. We expressed these glycoproteins using the bacterial expression system and analyzed recombinant proteins. Expression of glycoproteins gC and gD were observed on SDS-PAGE or Western blot analysis, but gB was not. Optimal concentration of IPTG and inducing time were determined as 1.0 mM and 4 h, respectively, for the expression of both gC and gD in E. coli. A sodium dodecyl sulfate (SDS) was the most efficient detergent in solubilizing insoluble recombinant protein. |
| Databáze: | OpenAIRE |
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