Accumulation of human EGF in nectar of transformed plants of Nicotiana langsdorffii × N. sanderae and transfer to honey by bees
| Autor: | W. K. Bleeker, M. Verbeek, A. A. Te Velde, Paulus H. S. Kwakman, L. C. P. Keizer, J. P. F. G. Helsper, Sebastiaan A. J. Zaat, J. Creemers-Molenaar, C. P. Ruyter-Spira, J. B. Bade |
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| Rok vydání: | 2011 |
| Předmět: |
Signal peptide
chemistry.chemical_classification fungi food and beverages Biological activity Peptide Plant Science General Medicine Biology biology.organism_classification In vitro Biochemistry chemistry Epidermal growth factor Botany Nectar Nicotiana langsdorffii Ecology Evolution Behavior and Systematics Nicotiana |
| Zdroj: | Plant Biology. 13:740-746 |
| ISSN: | 1435-8603 |
| DOI: | 10.1111/j.1438-8677.2010.00440.x |
| Popis: | Honey has been used successfully in wound healing for thousands of years. The peptide hormone human epidermal growth factor (hEGF) is also known to have a beneficial effect in various wound healing processes via mechanisms that differ from those for honey. In this study, we show that hEGF can be incorporated into honey via nectar. Plants of Nicotiana langsdorffii x N. sanderae were transformed with the gene for hEGF, equipped with a nectary-targeted promoter and a signal sequence for secretion to nectar. These plants accumulated hEGF in the nectar. The maximum hEGF concentration recorded with ELISA in these plants is 2.5 ng·ml⁻¹. There is a significant linear relationship (P |
| Databáze: | OpenAIRE |
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