Cover Picture: Semisynthesis and Characterization of the First Analogues of Pro-Neuropeptide Y (ChemBioChem 5/2003)

Autor:	Regula von Eggelkraut-Gottanka, Annette G. Beck-Sickinger, Zuzana Machova, Eric Grouzmann
Rok vydání:	2003
Předmět:	Organic Chemistry Native chemical ligation Neuropeptide Y receptor Biochemistry Combinatorial chemistry Chemical synthesis Semisynthesis law.invention chemistry.chemical_compound Solid-phase synthesis Biosynthesis chemistry law Peptide synthesis Recombinant DNA Molecular Medicine Molecular Biology
Zdroj:	ChemBioChem. 4:357-357
ISSN:	1439-4227
DOI:	10.1002/cbic.200390063
Popis:	The cover picture shows how a combination of recombinant synthesis and chemical synthesis has been used to obtain chemically modified proteins. N-terminal protein segments of pro-neuropeptide Y (proNPY) were produced as intein-fusion proteins in Escherischia coli in order to obtain thioesters. C-terminal segments were synthesized by parallel automated peptide synthesis and derivatized to obtain carboxyfluorescein- (CF) and biotin-labeled peptides. Native chemical ligation yielded chemically modified full-length analogues of proNPY that can be used to monitor the biosynthesis of neuropeptide Y. Futher information can be found in the article by Beck-Sickinger and co-workers on p. 425 ff.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1ceb4e3d66364d905e36fd50e6824d09 https://doi.org/10.1002/cbic.200390063 Zobrazit plný text záznamu Plný text