Cell Culture Bioassay to Evaluate Allelochemical Toxicity to Heliothis virescens (Lepidoptera: Noctuidae)

Autor:	Marcel H. Elissalde, James O. Norman, Robert D. Stipanovic, David W. Altman
Rok vydání:	1990
Předmět:	Ecology Heliothis virescens Caryophyllene General Medicine Biology biology.organism_classification Terpene Tissue culture chemistry.chemical_compound chemistry Gossypol Insect Science Botany Toxicity Noctuidae Bioassay Food science
Zdroj:	Journal of Economic Entomology. 83:737-741
ISSN:	1938-291X 0022-0493
Popis:	An insect tissue culture bioassay was developed with an established cell line (BCIRL-HV-AM1) of Heliothis virescens (F.). This bioassay substantially reduced the time, material, and experimental error involved in toxicity evaluations compared with larvalfeeding studies with artificial diets. LD50’S of seven terpenes from the cotton plant ( Gossypium spp.) were determined in the tissue culture bioassay. Various levels of toxicity were observed. Gossypol, hemigossypolone. and heliocides H1, H2 and H3 had LD50’S of 10 to 16 μg/ml. For caryophyllene oxide and caryophyllene, LD50’S were 53 μg/ml and 221 μg/ml, respectively. Comparison of these values with ED50’s obtained in larval-feeding studies validate the cell bioassayas an effective in vitro assay for relative toxicity. Twelve other terpenes of unknown toxicity to H. virescens also were evaluated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1ce672d1b549f36eeacd6b7b3557ce5c https://doi.org/10.1093/jee/83.3.737 Zobrazit plný text záznamu