Expression of protein arginine methyltransferase-5 in oral squamous cell carcinoma and its significance in epithelial-to-mesenchymal transition
| Autor: | Yoshiyuki Mori, Tomoko Tamura, Toshiro Niki, Daisuke Matsubara, Hiroshi Nishino, Taichiro Yoshimoto, Yusuke Amano |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Epithelial dysplasia Pathology medicine.medical_specialty Methyltransferase biology Chemistry Carcinoma in situ Protein arginine methyltransferase 5 Vimentin General Medicine medicine.disease Pathology and Forensic Medicine stomatognathic diseases 03 medical and health sciences Cytokeratin 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Cancer cell medicine Cancer research biology.protein Epithelial–mesenchymal transition |
| Zdroj: | Pathology International. 68:359-366 |
| ISSN: | 1320-5463 |
| DOI: | 10.1111/pin.12666 |
| Popis: | Protein arginine methyltransferases (PRMT) 5, a member of type II arginine methyltransferases, catalyzes the symmetrical dimethylation of arginine residues on histone and non-histone substrates. Although the overexpression of PRMT5 has been reported in various cancers, its role in oral squamous cell carcinoma (OSCC) has not been elucidated. In the present study, we immunohistochemically examined the expression of PRMT5 in surgically resected oral epithelial dysplasia (OED, n = 8), oral intraepithelial neoplasia (OIN)/carcinoma in situ (CIS) (n = 11) and OSCC (n = 52) with or without contiguous OED lesions. In the normal epithelium, PRMT5 was weakly expressed in the cytoplasm of basal layer cells. In OED, OIN/CIS, and OSCC, its expression consistently and uniformly increased in the cytoplasm of dysplastic and cancer cells. Moreover, nuclear and cytoplasmic localization was detected in the invasive front of cancer cells, particularly in cases showing poor differentiation or aggressive invasion patterns. The concomitant nuclear and cytoplasmic expression of PRMT5 correlated with the loss of E-cadherin and cytokeratin 17, and the upregulation of vimentin, features that are both indicative of epithelial-to-mesenchymal transition. PRMT5 may play a role from early oncogenesis through to the progression of OSCC, particularly in the aggressive mode of stromal invasion. |
| Databáze: | OpenAIRE |
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