| Autor: |
Chihiro Sugimoto, Kazuhiko Ohashi, Takeshi Matsumura, Misao Onuma, Noriko Itchoda, Kenji Ohya |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Plant Biotechnology 2002 and Beyond ISBN: 9789048162208 |
| DOI: |
10.1007/978-94-017-2679-5_79 |
| Popis: |
Type I interferon (IFN- α/β) is the first cytokine used for clinical applications against viral and neoplasmic diseases. Usually it is administrated by subcutaneous and intramuscular injection, but several studies have reported that orally administered Type I LFN is also effective against viral and autoimmune diseases. Therefore, we examined whether orally administered human IFN (HuIFN)-α can augment protection against systemic bacterial infection using Listeria monocytogenes infection in mice as an experimental model. Daily oral administrations for 6 days of 1000 international units (IU) of purified natural HulFN-α reduced bacterial burden in spleen and liver from L. monogtogenes-infected mice. This effect was observed in the middle phase of L. monocytogenes infection, but not in the early phase of the infection. Effects of oral administration of HuIFN-α expressed in potato plant were also examined in this infection model. Daily oral administrations of extracts of the transgenic potato tuber for 6 days decreased bacterial burden in the spleen. Lower doses of HuIFN-α in the extracts (20 IU/mouse/day) exerted a protective effect at almost the same level as the results achieved by the administration of 1000 IU of HuIFN-α. This result may be due to the ‘bioencapsulation’ effect for HuIFN-α by plant compartmentalization, which is one of the advantages of the plant expression system over other expression systems of recombinant proteins. Our present observation indicates the transgenic plants expressing cytokines can be used as feed/food and their additives in order to enhance natural immune responses in humans and animals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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