Concurrent overexpression of amino acid permease
| Autor: | Paula E. Jameson, J. E. Grant, Annu Smitha Ninan, Ondřej Novák, Martin Shaw, Natalia Cripps-Guazzone, Jiancheng Song, Mechthild Tegeder, Ivan Petřík |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Sucrose food.ingredient food and beverages Plant Science Biology 01 natural sciences Amino acid Cell wall Amino acid permease 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Invertase food chemistry Biochemistry Cytokinin Amino acid transporter Agronomy and Crop Science Cotyledon 010606 plant biology & botany |
| Zdroj: | Functional Plant Biology. 48:889-904 |
| ISSN: | 1445-4416 1445-4408 |
| DOI: | 10.1071/fp21011 |
| Popis: | Using pea as our model crop, we sought to understand the regulatory control over the import of sugars and amino acids into the developing seeds and its importance for seed yield and quality. Transgenic peas simultaneously overexpressing a sucrose transporter and an amino acid transporter were developed. Pod walls, seed coats, and cotyledons were analysed separately, as well as leaves subtending developing pods. Sucrose, starch, protein, free amino acids, and endogenous cytokinins were measured during development. Temporal gene expression analyses (RT-qPCR) of amino acid (AAP), sucrose (SUT), and SWEET transporter family members, and those from cell wall invertase, cytokinin biosynthetic (IPT) and degradation (CKX) gene families indicated a strong effect of the transgenes on gene expression. In seed coats of the double transgenics, increased content and prolonged presence of cytokinin was particularly noticeable. The transgenes effectively promoted transition of young sink leaves into source leaves. We suggest the increased flux of sucrose and amino acids from source to sink, along with increased interaction between cytokinin and cell wall invertase in developing seed coats led to enhanced sink activity, resulting in higher cotyledon sucrose at process pea harvest, and increased seed number and protein content at maturity. |
| Databáze: | OpenAIRE |
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