132 Impact of sticky end length on the diffraction of self-assembled DNA crystals
| Autor: | Yoel P. Ohayon, Rutu C. Shah, Victoria T. Adesoba, Esra Demirel, Matthew Lehmann, Ruojie Sha, Paul Chaikin, Jens J. Birktoft, Arun Richard Chandrasekaran, Sabrine I. Obbad, Nadrian C. Seeman |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Journal of Biomolecular Structure and Dynamics. 31:84-85 |
| ISSN: | 1538-0254 0739-1102 |
| DOI: | 10.1080/07391102.2013.786374 |
| Popis: | Our laboratory has reported a self-assembled 3-D crystal based on a DNA tensegrity triangle. The tensegrity triangle is a rigid DNA motif with three-fold rotational symmetry consisting of three helices whose axes are directed along three linearly independent directions (1). The triangles form a crystalline lattice stabilized via sticky ends (2). The length of the sticky ends reported previously was two nucleotides (nt) GA:TC. Although diffracting to 4 A resolution at the APS-ID19 beam line, they diffract only to 4.9 A at the NSLS-X25 beam line. In the current study, we have analysed the effect of sticky end length and sequence on crystal formation and the resolution of the X-ray diffraction pattern on NSLS-X25. Tensegrity triangle motifs having 1-, 2-, and 3-nt sticky ends have all formed crystals. X-ray diffraction data from the same beam line revealed that the crystal resolution was somewhat better for the 2-nt sticky end having an AA:TT base pair (4.75 A) than GA:CT and CC:GG (8.0 A). Moreover, the 1-n... |
| Databáze: | OpenAIRE |
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