Investigation of the tridimensional ultrastructure of rat glomerular capillary endothelium by high-resolution scanning electron microscopy
| Autor: | Robert J. Temkin, T. Banoub, M. Silverman, P.J. Lea, M.J. Hollenberg |
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| Rok vydání: | 1990 |
| Předmět: | |
| Zdroj: | Proceedings, annual meeting, Electron Microscopy Society of America. 48:30-31 |
| ISSN: | 2690-1315 0424-8201 |
| DOI: | 10.1017/s0424820100157681 |
| Popis: | The principal applications of scanning electron microscopy (SEM) to renal ultrastructure have been in the study of the surface topography of various kidney cell types and their orientation and distribution in both health and disease. SEM study, however, has been limited in a major way by a lack of resolution sufficient to readily examine in detail and in three dimensions such features as glomerular basement membrane substructure and the structural organization at high magnification of the glomerular, capillary endothelium. Consequently, most of our current information about the 3D ultrastructure of rat kidney glomerulus has been obtained from transmission electron (TEM) micrographs obtained from thin sections cut at various planes followed by computer assisted, serial reconstructions. Recent advances in specimen preparation techniques and scanning electron microscope design have permitted ultrastructural examination of the glomerular capillary wall in three dimensions using high resolution scanning electron microscopy (HRSEM). Specimens in which the cytosol and cytoskeleton have been extracted, but cell membranes nuclear structures and organelles left in place, were studied using a Hitachi SEM with a resolution of approximately 3 nm. |
| Databáze: | OpenAIRE |
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