| Autor: |
Heitor Siffert Pereira de Souza, Igor D. P. Soares, Morgana T. Castelo-Branco, Maria Isabel D. Rossi, Sergio Augusto Lopes de Souza, Celeste C. Elia, Daiana V. Lopes, Bianca Gutfilen, Lea Mirian Barbosa da Fonseca, Alberto Schanaider |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Gastroenterology. 138:S-415 |
| ISSN: |
0016-5085 |
| DOI: |
10.1016/s0016-5085(10)61909-9 |
| Popis: |
Background & Aims: Inflammatory bowel diseases (IBD) are characterized by chronic intestinal inflammation due to the loss of immune tolerance againstmucosal antigens. Mesenchymal stromal cells were recently shown to possess immunomodulatory action with beneficial therapeutic effects in immune mediated disorders. We investigated the potential therapeutic action of subcutaneous adipose tissue (AT-MSCs) or bone marrow-derived mesenchymal stromal cells (BM-MSCs) in a model of IBD. Methods: Male Wistar rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis were treated with AT-MSCs or BM-MSCs through intraperitoneal injection after colonoscopic detection of inflammation, at day 4. Colonoscopic and histologic scores were evaluated. Inflammatory response was determined by measuring the levels of inflammatory cytokines in cultures of colon samples, by ELISA. Collagen fibers were stained with picric acid dye and the density of collagen deposition was evaluated using a computerized image analysis system. MSCs were labeled with Tc-99m and administered contralateral to the site of inflammation. Scans were taken 30 minutes and 2 hours after injection, and isolated colon scans were obtained after euthanasia. Results: Intra-peritoneal injection of AT-MSCs or BM-MSCs significantly reduced the endoscopic (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
