Molecular Cloning, Characterization and Expression of cDNA for Rabbit Brain Tissue Factor

Autor:	Renata Horton, William H. Konigsberg, Aruna Pawashe, Ronald R Bach, Michael D. Ezekowitz, Tsung-Chung Lin
Rok vydání:	1991
Předmět:	chemistry.chemical_classification Pathology medicine.medical_specialty biology Factor VII Hematology Molecular cloning Molecular biology Amino acid chemistry.chemical_compound Tissue factor chemistry Coagulation Polyclonal antibodies Complementary DNA biology.protein medicine Glycoprotein
Zdroj:	Thrombosis and Haemostasis. 66:315-320
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1646414
Popis:	SummaryTissue factor (TF) is a membrane anchored glycoprotein that initiates blood coagulation by forming a complex with circulating factor VII or VIIa. TF has been identified in atherosclerotic plaques and may possibly trigger thrombosis after spontaneous plaque rupture as seen in acute myocardial infarction or angioplasty. We have previously developed an atherosclerotic rabbit model for study of the acute and chronic outcomes following angioplasty. As a first step in developing inhibitors of TF, we have isolated and characterized a rabbit cDNA coding for the mature TF. The sequence comparison of rabbit TF cDNA with those of human and mouse TFs show considerable similarity at both the nucleotide and amino acid levels. The TF cDNA when expressed in E. coli demonstrates a procoagulant activity comparable to that of native rabbit brain TF. The TF activity can be blocked by a polyclonal antibody against rabbit TF.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1c96bcd028da01faa27b378af57e35cc https://doi.org/10.1055/s-0038-1646414 Zobrazit plný text záznamu