| Autor: |
Marianne Dingemanse, Wim Van Hartingsveldt, I. E. Mattern, Cees A.M.J.J. Van Den Hondel, Richard P. Oliver, Peter J. Punt, Peter H. Pouwels, Cora van Zeijl |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Extracellular Enzymes of Microorganisms ISBN: 9781468412765 |
| Popis: |
The filamentous fungus Aspergillus niger produces a number of extracellular enzymes as well as primary and secondary metabolites, which are of considerable industrial interest. To facilitate molecular-genetic studies on the regulation of gene expression and protein secretion in this organism, the availability of an efficient gene-transfer system is of paramount importance. In this paper we describe the development of two such systems. The first one is a homologous transformation system, with a vector containing the A. niger pyrG gene as a selectable marker. In the second system, a vector containing the hygromycin B resistance gene as a dominant selectable marker is used for transformation. This system is also applicable to A. nidulans. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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