Enzymatic Urea Sensor Based on Graphene Oxide/Titanium Dioxide Films Modified by Urease-Magnetic Beads

Autor: Siao-Jie Yan, You-Xiang Wu, Chih-Hsien Lai, Cian-Yi Wu, Jung-Chuan Chou, Si-Hong Lin, Yi-Hung Liao
Rok vydání: 2019
Předmět:
Zdroj: IEEE Transactions on Nanotechnology. 18:336-344
ISSN: 1941-0085
1536-125X
DOI: 10.1109/tnano.2019.2907225
Popis: In this paper, we proposed an enzymatic urea sensor using urease-magnetic beads (MBs) modified graphene oxide (GO)/titanium dioxide (TiO 2 ) composite layers. The urea sensor was prepared by screen-printing technology and radio frequency sputtering. TiO 2 sensing film was deposited onto polyethylene terephthalate substrate, which serves as an efficient matrix for immobilization of urease. Preparation of GO/TiO 2 films was completed after GO being deposited onto the TiO 2 film. Moreover, the characterization and sensing properties of the urea sensor were performed by energy dispersive spectrometer, scanning electron microscope, atomic force microscopy, electrochemical impedance spectroscopy, and voltage-time measurement system. The results indicated that the GO/TiO 2 urea sensor enhances its performance of average sensitivity, response time, and drift rate after modifying the sensing membrane though MBs.
Databáze: OpenAIRE