Intracellular Generation of Reactive Oxygen Species and DNA Damage in Escherichia Coli Mutants Exposed to Electromagnetic Field and X-Ray
| Autor: | Y. Yonezawa, J. Miyakoshi, H. Takebe, H. Nishioka |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species DNA damage DNA repair General Medicine Biology medicine.disease_cause Superoxide dismutase chemistry.chemical_compound chemistry Biochemistry Catalase medicine biology.protein Environmental Chemistry Waste Management and Disposal Escherichia coli Intracellular DNA Water Science and Technology |
| Zdroj: | Environmental Technology. 20:45-51 |
| ISSN: | 1479-487X 0959-3330 |
| DOI: | 10.1080/09593332008616791 |
| Popis: | In order to examine intracellular generation of reactive oxygen species (ROS) and DNA damage in bacterial cells exposed to extremely low frequency magnetic field (ELFMF) at 400mT and 50Hz and X-ray, ‘kat-sod Assay’ and ‘rec-lac Assay’ were performed using E. coli mutants which were deficient in ROS scavenging enzymes, such as superoxide dismutase and/or catalase, and DNA repair capacities. Both assays were developed by Nishioka H. and Hayashi S. (1995) and Nunoshiba T. and Nishioka H. (1991) and consisted of survival test and β-galactosidase test. X-irradiation induced a clear intracellular generation of ROS and DNA damage, indicating a possibility that intracellular generated ROS may play a partial role in DNA damage by X-ray. On the other hand, no remarkable effects of ELFMF were seen in the cells using by ‘kat-sod Assay’ and ‘rec-lac Assay’, showing that ELFMF did not generate ROS intracellularly and damage DNA. |
| Databáze: | OpenAIRE |
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