| Autor: |
Sidney Roberts, K. Von Hungen |
| Rok vydání: |
1978 |
| Předmět: |
|
| DOI: |
10.1016/b978-1-4832-8322-7.50068-6 |
| Popis: |
1 The activation of brain adenylate cyclase by Ca++ in association with a Ca++-dependent regulator protein (CDR) is described. Adenylate cyclase and CDR were solubilized with Lubrol-PX and then separated from each other with the use of DEAE-cellulose chromatography. 2 Solubilized adenylate cyclase was activated rapidly by Ca++ in the presence of CDR. This activation was readily reversed in the presence of an excess of EGTA. 3 Membrane-bound adenylate cyclase from rat brain exhibited striking regional differences in sensitivity to Ca++. These differences disappeared upon solubilization of the preparations. 4 A model is presented which proposes that cellular cyclic nucleotide levels are regulated by free Ca++ via a specific Ca++ receptor. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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