White Sturgeon as a Potential Vector of Infectious Hematopoietic Necrosis Virus

Autor: S. E. Lapatra, Terry S. McDowell, Ronald P. Hedrick, Gerald R. Jones, R. Schneider, K. A. Lauda
Rok vydání: 1995
Předmět:
Zdroj: Journal of Aquatic Animal Health. 7:225-230
ISSN: 1548-8667
0899-7659
DOI: 10.1577/1548-8667(1995)007<0225:wsaapv>2.3.co;2
Popis: Cell lines from white sturgeon Acipenser transmontanus were derived from peripheral blood cells, heart, and spleen. Incubated with infectious hematopoietic necrosis virus (IHNV) for 8 d at l5°C, these cell lines produced 0.7–53.2 plaque-forming units (PFU)/cell. Waterborne exposure of larval white sturgeons (60 d posthatch) to 106 PFU/mL of IHNV resulted in 10% mortality 5–6 d postinfection, with virus concentrations consistently greater than 105 PFU/g. A replicate group of larval white sturgeons that were sampled at different times post-IHNV exposure had no detectable virus at 24 h, but 72% of the fish had IHNV concentrations of 102-106 PFU/g when they were examined 2–9 d postinfection. Juvenile white sturgeons (mean weight, 35 g) immersed in or injected with IHNV exhibited no mortality, and virus was only detected immediately postexposure in just 25% of the fish tested. Juvenile white sturgeons fed either virus-free rainbow trout Oncorhynchus mykiss or dead IHNV-infected rainbow trout had no vi...
Databáze: OpenAIRE
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