Estimation of Alprazolam and Sertraline in Pure Powder and Tablet Formulations by High-Performance Liquid Chromatography and High-Performance Thin-Layer Chromatography
| Autor: | Arpita B. Patel, Kashyap K. Bhatt, Rashmin B. Patel, Mrunali R. Patel, M. B. Shankar |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Chromatography
Chemistry Silica gel Biochemistry (medical) Clinical Biochemistry Biochemistry Toluene High-performance liquid chromatography Thin-layer chromatography Analytical Chemistry chemistry.chemical_compound Electrochemistry Acetone Particle size High performance thin layer chromatography Acetonitrile Spectroscopy |
| Zdroj: | Analytical Letters. 42:1588-1602 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032710902994165 |
| Popis: | This article describes validated high-performance liquid chromatographic (HPLC) and high-performance thin-layer chromatographic (HPTLC) methods for simultaneous estimation of alprazolam (ALZ) and sertraline (SER) in pure powder and tablet formulation. The HPLC separation was achieved on a Nucleosil C18 column (150 mm long, 4.6 mm i.d., and 5-µm particle size) using acetonitrile and phosphate buffer (50 + 50 v/v), pH 5.5, as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60 F254 using acetone/toluene/ammonia (6.0:3.0:1.0, v/v/v) as the mobile phase. Quantification with the HPLC method was achieved with ultraviolet (UV) detection at 230 nm over the concentration range 3–18 µg/mL for both drugs with mean recovery of 101.86 ± 0.21 and 100.57 ± 0.31% for ALZ and SER, respectively. Quantification in HPTLC was achieved with UV detection at 230 nm over the concentration range of 400–1400 ng/spot for both dr... |
| Databáze: | OpenAIRE |
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